Magnification enlarges the image, whereas resolution separates two nearby objects.
📌 Visible transmitted light microscopy uses polychromatic light in air, whereas fluorescence microscopy uses a single monochromatic wavelength.
1. What does magnification measure in microscopy?
2. A microscope can produce a very large image, but two nearby points still appear as one blurred point. Which property is limiting the observation?
3. Which comparison correctly distinguishes visible transmitted light microscopy from fluorescence microscopy?
What is magnification in microscopy?
The ratio between observed dimension and actual object size.
What does the resolution limit define in microscopy?
The minimum distance to distinguish two objects.
What factors determine the resolution limit in microscopy?
Light diffraction, numerical aperture, and refractive index.
What type of light does visible transmitted light microscopy use?
Polychromatic light in air.
What type of light does fluorescence microscopy use?
A single monochromatic wavelength.
What is the resolution limit in visible light microscopy?
200 nanometers.
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