★ Must-know
Further detail
Isolate → examine → identify
1. What is the primary purpose of bacterial culture in a clinical laboratory?
2. Which finding represents a microscopic examination of a bacterial culture?
3. A bacterium is placed in a medium with adequate nutrients and water but a pH of 5.0. Which growth requirement is most clearly unsuitable?
What is the purpose of bacterial culture?
To obtain a pure bacterial isolate for examination and identification.
Which features can be examined in bacterial cultures?
Colony morphology, microscopic appearance, biochemical properties, antibiotic resistance, serotype, MALDI-TOF results, and PCR-detected genes.
Which bacteria are unculturable on nutrient media?
Mycobacterium leprae, Treponema pallidum, and obligate intracellular Chlamydia spp.
What pH range is optimal for bacterial growth?
An optimal pH of 7.2–7.4.
How are bacteria classified by their atmospheric requirements?
As aerobic, microaerophilic, capnophilic, obligately anaerobic, or facultatively anaerobic.
What is the optimal growth temperature range for mesophilic bacteria?
30–40°C.
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