Sterilization: The process of destructing or destroying all forms of microbial life, including highly resistant bacterial spores. (Source: Prepared by Dr. Helen Tang Hooi Chia)
Sterilant: A sterilizing agent used specifically in sterilization procedures. (Source: Prepared by Dr. Helen Tang Hooi Chia)
Disinfection: The process of eliminating or reducing most pathogenic microorganisms from inanimate objects and surfaces. (Source: Prepared by Dr. Helen Tang Hooi Chia)
Disinfectant: Chemicals used in disinfection to achieve microbial reduction. (Source: Prepared by Dr. Helen Tang Hooi Chia)
Antiseptic: Chemicals used to eliminate or reduce most pathogenic microorganisms on living tissues. (Source: Prepared by Dr. Helen Tang Hooi Chia)
Aseptic Technique: A broader protocol designed to maintain asepsis, ensuring objects or areas are free of pathogens. (Source: Prepared by Dr. Helen Tang Hooi Chia)
1. What does moist heat sterilization primarily refer to in microbial control?
2. What is the primary effect of dry heat sterilization on microorganisms?
3. When sterilizing heat-sensitive surgical instruments that cannot withstand moisture, which method should be applied in practice based on the concept of sterilization?
Sterilization — definition?
Kills all microorganisms, including spores.
Sterilization methods — classification?
Physical and chemical methods.
Dry heat sterilization — method?
Uses hot air or flame to kill microbes.
Moist heat sterilization — method?
Uses steam under pressure, e.g., autoclaving.
Physical sterilization techniques — examples?
Heat, radiation, filtration.
Moist heat methods — example?
Autoclaving at 121°C.
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